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THE EFFECT OF MECHANICAL FACTOR ON THE CELL DAMAGE UPON REWARMING.

[In Russian. / En russe.]

Author(s) : VOROTILIN A. M.

Type of article: Article

Summary

CHANGES IN THE PHYSICAL STATE AND PHASE TRANSITIONS IN CRYOPROTECTIVE MEDIA CONTAINING 1.2-PROPANEDIOL OR GLYCEROL AND THOSE WITH ADDED ERYTHROCYTES WERE STUDIED USING METHODS OF DIFFERENTIAL SCANNING CALORIMETRY AND THERMAL ANALYSI TRANSFORMATIONS CAUSED BY THERMAL EFFECTS AS WELL AS OTHER CHANGES IN THE STUDIED SYSTEMS HAVE BEEN SHOWN TO OCCUR AT TEMPERATURES HIGHER THAN THE DEVITRIFICATION POINT, NAMELY AT 153 TO 173 K (-120 TO -100 DEG C). THE CONTRIBUTION OF DETRIMENTAL EFFECT OF THERMAL AND MECHANICAL TENSIONS ON CELLS WAS ESTIMATED BY THEIR INTEGRITY AFTER THAWING AS A FUNCTION OF REWARMING RATES IN A RANGE OF 77 K (-196 DEG C) TO THE DEVITRIFICATION POINT WHERE THE MENTIONED TRANSFORMATIONS WERE NOT OBSERVED.

Details

  • Original title: [In Russian. / En russe.]
  • Record ID : 1989-1193
  • Languages: Russian
  • Source: Kriobiologija - n. 3
  • Publication date: 1988
  • Document available for consultation in the library of the IIR headquarters only.

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