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THE INVESTIGATION OF COMPARATIVE STABILITY OF AMOEBA PROTEUS NUCLEUS AND CYTOPLASM BY METHODS OF CYTOSURGERY AND ELECTRON MICROSCOPY.

Author(s) : KALININA L. V., GROMOV D. B.

Summary

AS HAD BEEN SHOWN BY METHODS OF CYTOSURGERY (ENUCLEATION, NUCLEAR TRANSFER) AND ELECTRON MICROSCOPY, THE AUTHORS FOUND THAT AMOEBA NUCLEI CAN BE PRESERVED BY FREEZING TO 77 K (-196 DEG C). THE NUCLEAR MORPHOLOGY AND ULTRASTRUCTURE APPEARED TO UNDERGO DAMAGE UPON RAPID COOLING OF CELLS TO 77 K, BUT INSIGNIFICANTLY. INDIVIDUAL PORE COMPLEXES OF THE NUCLEAR MEMBRANE SEEMED TO BE INJURED ; HOWEVER, THEY RECOVERED AT THE COST OF ENDOPLASMIC RETICULUM WHEN NUCLEI WERE TRANSFERRED TO ENUCLEATED CELLS. THE RESULTS PROVIDE PREREQUI-SITES FOR USING CYTOSURGICAL METHODS IN CRYOBIOLOGICAL STUDIES OF THOSE CELLS TO WHICH THEY ARE APPLICABLE, FOR EXAMPLE, EMBRYO CELLS.

Details

  • Original title: THE INVESTIGATION OF COMPARATIVE STABILITY OF AMOEBA PROTEUS NUCLEUS AND CYTOPLASM BY METHODS OF CYTOSURGERY AND ELECTRON MICROSCOPY.
  • Record ID : 1990-2155
  • Languages: English
  • Publication date: 1989/07/06
  • Source: Source: Proc. IVth int. Sch. Cryobiol. Freeze-drying, Borovets
    P27-P28; 1 tabl.; 6 ref.
  • Document available for consultation in the library of the IIR headquarters only.

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