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Ventilation of cryostorage facilities of tissue establishments.

Author(s) : LAIN M., MERICKA P., DVORÁK J.

Summary

The liquid nitrogen is commonly used for long-term storage of viable cells and tissues in the cryostorage facilities of tissue establishments. As frequently large biological containers are used the continuous evaporation of nitrogen during storage as well as evaporation during refilling containers can lead to the decrease of the air oxygen level below acceptable limits (20% for women, 18% for men - according to the Czech law and the recommendations of producers). For this reason sufficient ventilation of such facilities is essential for the safety of the staff. Ventilation should also eliminate the possible leakage of liquid nitrogen in extraordinary situations. The character of nitrogen production is non-stationary due to the above-mentioned situations. The authors present the basic model based on non-stationary pollutant production. The nitrogen production in the model is calibrated according to on-site measurements, and can be used for the prediction of specific operation and emergency scenarios. The principles and recommendations for cryogenic storage room ventilation systems, its control and environment monitoring equipment are also presented.

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Pages: 2008-1

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Details

  • Original title: Ventilation of cryostorage facilities of tissue establishments.
  • Record ID : 2008-2246
  • Languages: English
  • Source: Cryogenics 2008. Proceedings of the 10th IIR International Conference
  • Publication date: 2008/04/21

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