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VIABILITY OF RAM TESTICULAR SPERMATOZOA FOLLOWING CRYOPRESERVATION IN RETE TESTIS FLUID.

Author(s) : VOGLMAYR J. K., CHARTIER D. M., SAWYER R. F. Jr

Type of article: Article

Summary

RAM TESTICULAR SPERMATOZOA, COLLECTED CONTINUOUSLY FROM THE CANNULATED TESTIS, WERE FROZEN USING THE CRYOPROTECTIVE AGENTS EGG YOLK AND GLYCEROL. FREEZE-THAWING SIGNIFICANTLY DEPRESSED THE RESPIRATION RATE AND GLYCOLYTIC ACTIVITY OF TESTICULAR SPERMATOZOA. MORPHOLOGICALLY, THERE WAS LITTLE EVIDENCE OF CRYODAMAGE. SURFACE RADIOIODINATION OF FROZEN-THAWED TESTICULAR SPERMATOZOA WAS HIGHLY SELECTIVE. THESE RESULTS CONFIRM THAT TESTICULAR SPERMATOZOA HAVE A GREATER LOW-TEMPERATURE TOLERANCE THAN DO EJACULATED SPERMATOZOA AND THAT CRYOPRESERVATION OF IMMATURE TESTICULAR SPERMATOZOA IN RETE TESTIS FLUID WITH ADDED EGG YOLK AND GLYCEROL MAY BE A USEFUL APPROACH TO EXTEND THE AVAILABILITY OF THESE CELLS.

Details

  • Original title: VIABILITY OF RAM TESTICULAR SPERMATOZOA FOLLOWING CRYOPRESERVATION IN RETE TESTIS FLUID.
  • Record ID : 1984-1249
  • Languages: English
  • Source: Cryobiology - vol. 20 - n. 4
  • Publication date: 1983

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