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A SCANNING ELECTRON MICROSCOPE STUDY OF CRYOPRESERVED PANCREATIC BETA CELLS.

Author(s) : WISE M.

Type of article: Article

Summary

ISLETS OF LANGERHANS ISOLATED FROM PORCINE PANCREAS WERE EXPOSED TO DMSO AT 273 K (0 DEG C) AND FROZEN IN LIQUID NITROGEN. AFTER REWARMING THE DMSO WAS REMOVED FROM THE ISLETS USING A PROTOCOL OF SERIAL DILUTIONS OF SUCROSE TO MAINTAIN OSMOTIC STABILITY, OR A LARGE VOLUME OF UNMODIFIED BASAL MEDIUM EAGLES CULTURE MEDIUM. THE CONSEQUENCES ON BOTH ENDOCRINE AND EXOCRINE CELLS WERE EXAMINED BY SCANNING ELECTRON MICROSCOPY.

Details

  • Original title: A SCANNING ELECTRON MICROSCOPE STUDY OF CRYOPRESERVED PANCREATIC BETA CELLS.
  • Record ID : 1984-2477
  • Languages: English
  • Source: Cryo-Letters - vol. 5 - n. 1
  • Publication date: 1984
  • Document available for consultation in the library of the IIR headquarters only.

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