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CRYOPRESERVATION OF RABBIT CORNEAS BY COMBINATION OF DEXTRAN-40, HUMAN ALBUMIN AND LOW DOSES OF DMSO: A SCANNING ELECTRON MICROSCOPY STUDY.

Author(s) : NERONOV A. J., GORANOV I. T., MIHAILOVA-BOIADJIEVA A.

Type of article: Article

Summary

THE INFLUENCE OF DIFFERENT CONCENTRATIONS OF DMSO AND DEXTRAN-40 ON THE PRESERVATION OF THE ENDOTHELIAL LAYER OF RABBIT CORNEA WAS INVESTIGATED. THE EFFECT OF INCLUDING HUMAN ALBUMIN WAS ASSESSED. THE INTEGRITY OF THE ENDOTHELIAL LAYER WAS STUDIED BY SCANNING ELECTRON MICROSCOPY. EVALUATION OF THE SURVIVAL OF INTACT ENDOTHELIAL CELLS SHOWED THAT 87 MORE OR LESS 8% WAS ACHIEVED USING 4% DMSO WITH 8% DEXTRAN-40 AND A COOLING RATE OF 1 K/MIN. SLIGHTLY IMPROVED RECOVERY (96 MORE OR LESS 3%) WAS OBTAINED WHEN 20% HUMAN ALBUMIN WAS INCLUDED IN THE CRYOPROTECTANT COMBINATION.

Details

  • Original title: CRYOPRESERVATION OF RABBIT CORNEAS BY COMBINATION OF DEXTRAN-40, HUMAN ALBUMIN AND LOW DOSES OF DMSO: A SCANNING ELECTRON MICROSCOPY STUDY.
  • Record ID : 1990-2150
  • Languages: English
  • Source: Cryo-Letters - vol. 10 - n. 5
  • Publication date: 1989/09
  • Document available for consultation in the library of the IIR headquarters only.

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