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Antilisterial activity of peptide AS-48 and study of changes induced in the cell envelope properties of an AS-48-adapted strain of Listeria monocytogenes.

Author(s) : MENDOZA F., MAQUEDA M., GÁLVEZ A., et al.

Type of article: Article

Summary

The peptide AS-48 is highly active on all Listeria species. It has a bactericidal and bacteriolytic mode of action on Listeria monocytogenes CECT 4032, causing depletion of the membrane electrical potential and pH gradient. The producer strain Enterococcus faecalis A-48-32, releases sufficient amounts of AS-48 into the growth medium to suppress L. monocytogenes in cocultures at enterococcus-to-listeria ratios above 1 at 37 °C or above 10 at 15 °C. As the temperature decreases, the bactericidal effects of AS-48 are less pronounced but 2.5 µg/ml it still can inhibit the growth of Listeria at 6 °C. AS-48 adapted cells can be selected at low (but still inhibitory) concentrations, and they can be inhibited completely by AS-48 at 0.5 µg/ml. AS-48ad cells are cross-resistant to nisin and show an increased resistance to muramidases. Fatty acid composition of adapted cells is modified: they show a much higher proportion of branched fatty acids.

Details

  • Original title: Antilisterial activity of peptide AS-48 and study of changes induced in the cell envelope properties of an AS-48-adapted strain of Listeria monocytogenes.
  • Record ID : 2000-0758
  • Languages: English
  • Source: Appl. environ. Microbiol. - vol. 65 - n. 2
  • Publication date: 1999/02

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