Chill sensitivity and cryoprotectant permeability of dechorionated zebrafish embryos, Brachydanio rerio.

Author(s) : HAGEDORN M., KLEINHANS F. W., WILDT D. E., RALL W. F.

Type of article: Article

Summary

The zebrafish (Brachydanio rerio) was used as a model for basic studies of the chilling sensitivity, permeability and toxicity of cryoprotectants. The permeability of dechorionated embryos to cryoprotectants was examined by measuring the kinetics of volumetric change at various developmental stages. The authors conclude that the complex nature of the zebrafish embryo reduces the effectiveness and predictive value of light microscopical measurements for cryoprotectant permeability studies.

Details

  • Original title: Chill sensitivity and cryoprotectant permeability of dechorionated zebrafish embryos, Brachydanio rerio.
  • Record ID : 1998-3193
  • Languages: English
  • Source: Cryobiology - vol. 34 - n. 3
  • Publication date: 1997/05

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