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Studies on chilling sensitivity of zebrafish (Brachydanio rerio) embryos.

Author(s) : ZHANG T., RAWSON D. M.

Type of article: Article

Summary

10 developmental stages of zebrafish embryos were studied for chilling sensitivity. Early developmental stages were the most sensitive to chilling. Heartbeat-stage embryos tolerated a temperature of 0 deg C for up to 10 hours without adverse effect, but embryo survival was significantly reduced at subzero temperatures. 27- to 40-hour stage embryos showed sensitivity to chilling; 45- to 49-hour (prehatch) stage embryos showed increased sensitivity. Cryoprotective effects of various media on chilling sensitivity are studied as well. The presence of sucrose or trehalose slightly enhanced cooling tolerance of the embryos. Methanol proved to be an optimal cryoprotectant for nonfreezing storage of embryos at zero and subzero temperatures.

Details

  • Original title: Studies on chilling sensitivity of zebrafish (Brachydanio rerio) embryos.
  • Record ID : 1996-1217
  • Languages: English
  • Source: Cryobiology - vol. 32 - n. 3
  • Publication date: 1995/06

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