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Effect of different concentrations of cryoprotectant and extender on the hatching of Indian major carp embryos (Labeo rohita, Catla catla, and Cirrhinus mrigala) stored at low temperature.

Author(s) : AHAMMAD M. M., BHATTACHARYYA D., JANA B. B.

Type of article: Article

Summary

Rohu (Labeo rohita), catla (Catla catla) and mrigal (Cirrhinus mrigala) embryos were examined for hatching success after short-term (8-13 h) storage at low temperature (4 deg C) using three concentrations of three cryoprotectants, methanol, dimethyl sulfoxide, and glycerol, separately as well as together with a single concentration of sucrose (0.5 M). The aim was to determine the toxicity of cryoprotectants for fish embryos at 4 deg C. Of the cryoprotectants used, methanol was found to be most suitable for low-temperature storage in the refrigerator. The hatching rate of embryos was significantly higher when sucrose was added to methanol when compared with methanol alone. Both rohu and catla embryos survived with either 1 or 2 M methanol, with or without the addition of sucrose, but the addition of sucrose was essential for survival of mrigal at 1 and 2 M methanol.

Details

  • Original title: Effect of different concentrations of cryoprotectant and extender on the hatching of Indian major carp embryos (Labeo rohita, Catla catla, and Cirrhinus mrigala) stored at low temperature.
  • Record ID : 1999-3898
  • Languages: English
  • Source: Cryobiology - vol. 37 - n. 4
  • Publication date: 1998/12

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