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Cold- and cryopreservation of dog liver and kidney slices.

Author(s) : FISHER R. L., HASAL S. J., SANUIK J. T., HASAL K. S., GANDOLFI A. J., BRENDEL K.

Type of article: Article

Summary

Dog liver and kidney slices were placed in cold storage at 0 deg C using Viaspan (UW), Euro-Collins (EC), Sacks + prostacyclin (SP), and V-7 (V7) cold preservation solutions for 10 days. Dog liver slices can be cold-preserved in V7 for up to 7 days using potassium ion retention as the viability criterion but only up to 4 days using protein synthesis. Dog kidney slices can be cold-preserved in UW, EC, and V7 for up to 10 days using potassium ion retention, but only V7 could maintain protein synthesis for 10 days. Cryopreserved dog liver and kidney slices retained 63-68% of control viability after 4 hours of incubation in fetal calf serum.

Details

  • Original title: Cold- and cryopreservation of dog liver and kidney slices.
  • Record ID : 1997-0553
  • Languages: English
  • Source: Cryobiology - vol. 33 - n. 1
  • Publication date: 1996/02

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