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Cold- and cryopreservation of human liver and kidney slices.

Author(s) : FISHER R. L., HASAL S. J., SANUIK J. T., SCOTT K. S., GANDOLFI A. J., BRENDEL K.

Type of article: Article

Summary

Human liver and kidney slices were cold stored for 10 days in Belzers University of Wisconsin, Euro-Collins, and modified Sacks solutions. Another set of slices was cryopreserved at 1 deg C/min for liver and 12 deg C/min for kidney using a 10% dimethyl sulfoxide/fetal calf serum (FCS) cryoprotectant solution. The cold- and cryopreserved slices were incubated in roller culture for 4 hours using FCS as the media. Following cryopreservation, human liver slice viability was retained at between 65 and 90% of control values, while kidney slice viability was maintained between 70 and 90% of control values depending on the viability parameter. These results indicate that this human in vitro tissue slice system can be used to optimize preservation solutions and methods.

Details

  • Original title: Cold- and cryopreservation of human liver and kidney slices.
  • Record ID : 1994-1873
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 3
  • Publication date: 1993/06

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