Share

CRYOBIOLOGICAL PROCESSES AND THEIR APPLICATIONS: SOME EXAMPLES.

LES TECHNIQUES DE CRYOBIOLOGIE ET LEURS APPLICATIONS : QUELQUES EXEMPLES.

Author(s) : ANGELIER N.

Type of article: Article

Summary

RED BLOOD CELLS ARE FROZEN AND STORED IN PLASTIC BAGS, IN LIQUID NITROGEN, AFTER ADDITION OF GLYCEROL. THEIR CLINICAL INDICATIONS, AFTER THAWING AND WASHING, ARE SURVEYED. THE AUTHOR DETAILS THE FREEZING PROCESSES (WITH A PROGRAMMABLE FREEZER) FOR BONE MARROW IN PLASTIC BAGS, AFTER PURIFICATION OF MARROW CELLS AND ADDITION OF A CRYOPROTECTANT (DMSO), REPORTING THE ADVANTAGE OF BONE MARROW AUTOGRAFTS FOR THE TREATMENT OF LEUKAEMIA AND OTHER MALIGNANT HAEMOPATHIES. THE STAGES OF SEMEN CRYOPRESERVATION ARE AS FOLLOWS: COLLECTION, LIQUEFACTION, DILUTION WITH A CRYOPROTECTANT INCLUDING GLYCEROL, PACKING IN STRAWS, FREEZING AND STORAGE IN LIQUID NITROGEN. THE INTEREST OF FRENCH INVESTIGATIONS INTO THE FREEZING OF BOVINE EMBRYOS IS STRESSED: USE OF A PROGRAMMABLE FREEZER FOR THE SLOW COOLING OF EMBRYOS, ADDED WITH GLYCEROL, FROM 266 TO 123 K (-7 TO -150 DEG C). G.G.

Details

  • Original title: LES TECHNIQUES DE CRYOBIOLOGIE ET LEURS APPLICATIONS : QUELQUES EXEMPLES.
  • Record ID : 1984-1228
  • Languages: French
  • Source: Rev. gén. Froid - vol. 73 - n. 7
  • Publication date: 1983
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (7)
See the source

Indexing