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CRYOPRESERVATION OF CASSAVA ZYGOTIC EMBRYOS AND WHOLE SEEDS IN LIQUID NITROGEN.

Author(s) : MARIN M. L.

Type of article: Article

Summary

CRYOPRESERVATION IN LIQUID NITROGEN OF ISOLATED ZYGOTIC EMBRYOS AND WHOLE SEEDS OF CASSAVA HAVE BEEN STUDIED. A SIMPLE METHODIS PROPOSED FOR UTILIZATION IN GERMPLASM CONSERVATION. VERY HIGH PERCENTAGE SURVIVAL VALUES (ABOUT 97%) OF ZYGOTIC EMBRYOS AND WHOLE SEEDS WERE OBTAINED BY BOTH SLOW AND RAPID COOLING METHODS FOLLOWED BY SLOW THAWING: 1 HOUR AT 203 K (-70 DEG C) FOLLOWED BY 1 HOUR AT 258 K (-15 DEG C). IN THE CASE OF WHOLE SEEDS, SLOW THAWING PREVENTED SHATTERING.

Details

  • Original title: CRYOPRESERVATION OF CASSAVA ZYGOTIC EMBRYOS AND WHOLE SEEDS IN LIQUID NITROGEN.
  • Record ID : 1991-1184
  • Languages: English
  • Source: Cryo-Letters - vol. 11 - n. 4
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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