ShareCRYOPRESERVATION OF CASSAVA ZYGOTIC EMBRYOS AND WHOLE SEEDS IN LIQUID NITROGEN.
Author(s) : MARIN M. L.
Type of article: Article
Summary
CRYOPRESERVATION IN LIQUID NITROGEN OF ISOLATED ZYGOTIC EMBRYOS AND WHOLE SEEDS OF CASSAVA HAVE BEEN STUDIED. A SIMPLE METHODIS PROPOSED FOR UTILIZATION IN GERMPLASM CONSERVATION. VERY HIGH PERCENTAGE SURVIVAL VALUES (ABOUT 97%) OF ZYGOTIC EMBRYOS AND WHOLE SEEDS WERE OBTAINED BY BOTH SLOW AND RAPID COOLING METHODS FOLLOWED BY SLOW THAWING: 1 HOUR AT 203 K (-70 DEG C) FOLLOWED BY 1 HOUR AT 258 K (-15 DEG C). IN THE CASE OF WHOLE SEEDS, SLOW THAWING PREVENTED SHATTERING.
Details
- Original title: CRYOPRESERVATION OF CASSAVA ZYGOTIC EMBRYOS AND WHOLE SEEDS IN LIQUID NITROGEN.
- Record ID : 1991-1184
- Languages: English
- Source: Cryo-Letters - vol. 11 - n. 4
- Publication date: 1990
- Document available for consultation in the library of the IIR headquarters only.
Links
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Indexing
- Themes: Seeds and plants
- Keywords: Cassava; Survival; Seed; Embryo; Cryopreservation
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- Date : 1992/09
- Languages : English
- Source: Cryo-Letters - vol. 13 - n. 5
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PRELIMINARY OBSERVATIONS ON THE CRYOPRESERVATIO...
- Author(s) : CHAUDHURY R.
- Date : 1991
- Languages : English
- Source: Cryo-Letters - vol. 12 - n. 1
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The sequence of the preparative procedures affe...
- Author(s) : STEWART P., TAYLOR M., MYCOCK D.
- Date : 2001/01
- Languages : English
- Source: CryoLetters - vol. 22 - n. 1
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Seed storage and cryoexposure behaviour in haze...
- Author(s) : NORMAH M. N., REED B. M., YU X.
- Date : 1994/09
- Languages : English
- Source: Cryo-Letters - vol. 15 - n. 5
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Cryopreservation of embryonic axes of almond se...
- Author(s) : CHAUDHURY R., CHANDEL K. P. S.
- Date : 1995/01
- Languages : English
- Source: Cryo-Letters - vol. 16 - n. 1
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