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Cryopreservation of Chymomyza larvae (Diptera: Drosophilidae) at -196 deg C with extracellular freezing.

Author(s) : MOON I., FUJIKAWA S., SHIMADA K.

Type of article: Article

Summary

Ice-inoculation at -2 deg C, cooling at 0.1 deg C/minute to -40 deg C and holding overnight for complete extracellular ice formation were followed by immersion in liquid nitrogen. About 60% of cold-acclimated diapausing larvae survived after 1-hour preservation at -196 deg C and developed into pupae. Non-diapausing larvae were less tolerant to freezing than diapausing larvae. No ice crystals were found within the larval tissues. The present results suggest that not only the vitrification protocol but also a conventional slow freezing method are useful for the preservation of this drosophilid species at cryogenic temperature.

Details

  • Original title: Cryopreservation of Chymomyza larvae (Diptera: Drosophilidae) at -196 deg C with extracellular freezing.
  • Record ID : 1997-0542
  • Languages: English
  • Source: Cryo-Letters - vol. 17 - n. 2
  • Publication date: 1996/03
  • Document available for consultation in the library of the IIR headquarters only.

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