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A simple method for the cryopreservation of axenic strains of Naegleria gruberi has been developed, using non-specialized equipment and a three-step freezing protocol. Cells from trophozoite stage, log-phase, cultures were transferred to medium containing 5% (v/v) DMSO, then placed in a series of refrigerators: -29 deg C (2 hours); -70 deg C (2 hours); liquid nitrogen -196 deg C. Viability levels of higher than 40% were achieved for all strains tested, and high viability levels were maintained on storage for 2 years under liquid nitrogen.
Details
- Original title: An improved method for the long-term preservation of Naegleria gruberi.
- Record ID : 1994-1859
- Languages: English
- Source: Cryo-Letters - vol. 14 - n. 6
- Publication date: 1993/11
- Document available for consultation in the library of the IIR headquarters only.
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Long-term cryopreservation of Toxoplasma gondii.
- Author(s) : POPESCU C., STERIU D.
- Date : 1993/11
- Languages : English
- Source: Cryo-Letters - vol. 14 - n. 6
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A SIMPLE TECHNIQUE FOR CRYOPRESERVATION OF THE ...
- Author(s) : MARCIN A.
- Date : 1989/03
- Languages : English
- Source: Cryo-Letters - vol. 10 - n. 2
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CRYOPRESERVATION OF GIARDIA LAMBLIA WITH DIMETH...
- Author(s) : LYMAN J. R., MARCHIN G. L.
- Date : 1984
- Languages : English
- Source: Cryobiology - vol. 21 - n. 2
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Cryopreservation of zoophthora radicans (Zygomy...
- Author(s) : SANDSKAR B., MAGALHAES B.
- Date : 1994/04
- Languages : English
- Source: Cryobiology - vol. 31 - n. 2
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PRESERVATION OF BACTERIAL L-FORMS BY FREEZING.
- Author(s) : TAKAHASHI T.
- Date : 1980
- Languages : English
- Source: Jap. J. exp. Med. - vol. 50 - n. 4
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