ShareLong-term cryopreservation of Toxoplasma gondii.
Author(s) : POPESCU C., STERIU D.
Type of article: Article
Summary
Optimal cryoprotection conditions for Toxoplasma gondii were investigated. The highest level of cell survival was obtained with a continuous slow cooling of 0.2-0.3 deg C/min from 24 to 20 deg C and 1 deg C/min to -60 deg C followed by immersion in liquid nitrogen with 8-10% (v/v) DMSO in RPMI 1640 medium, supplemented with 20 milliM glutamine and fast thawing in 0.5 M sorbitol at 37 deg C.
Details
- Original title: Long-term cryopreservation of Toxoplasma gondii.
- Record ID : 1994-1860
- Languages: English
- Source: Cryo-Letters - vol. 14 - n. 6
- Publication date: 1993/11
- Document available for consultation in the library of the IIR headquarters only.
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An improved method for the long-term preservati...
- Author(s) : BROWN S., DAY J. G.
- Date : 1993/11
- Languages : English
- Source: Cryo-Letters - vol. 14 - n. 6
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A SIMPLE TECHNIQUE FOR CRYOPRESERVATION OF THE ...
- Author(s) : MARCIN A.
- Date : 1989/03
- Languages : English
- Source: Cryo-Letters - vol. 10 - n. 2
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CRYOPRESERVATION OF GIARDIA LAMBLIA WITH DIMETH...
- Author(s) : LYMAN J. R., MARCHIN G. L.
- Date : 1984
- Languages : English
- Source: Cryobiology - vol. 21 - n. 2
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Cryopreservation of zoophthora radicans (Zygomy...
- Author(s) : SANDSKAR B., MAGALHAES B.
- Date : 1994/04
- Languages : English
- Source: Cryobiology - vol. 31 - n. 2
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Cryopreservation of living organs.
- Author(s) : TANASAWA I., NAGATA S., KIMURA N.
- Date : 1993
- Languages : Japanese
- Source: Trans. JAR - vol. 10 - n. 1
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