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Long-term cryopreservation of Toxoplasma gondii.

Author(s) : POPESCU C., STERIU D.

Type of article: Article

Summary

Optimal cryoprotection conditions for Toxoplasma gondii were investigated. The highest level of cell survival was obtained with a continuous slow cooling of 0.2-0.3 deg C/min from 24 to 20 deg C and 1 deg C/min to -60 deg C followed by immersion in liquid nitrogen with 8-10% (v/v) DMSO in RPMI 1640 medium, supplemented with 20 milliM glutamine and fast thawing in 0.5 M sorbitol at 37 deg C.

Details

  • Original title: Long-term cryopreservation of Toxoplasma gondii.
  • Record ID : 1994-1860
  • Languages: English
  • Source: Cryo-Letters - vol. 14 - n. 6
  • Publication date: 1993/11
  • Document available for consultation in the library of the IIR headquarters only.

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