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CRYOPRESERVATION OF HUMAN MONOCYTES.

Author(s) : MEULEN F. W. van der

Type of article: Article

Summary

MONOCYTES WERE ISOLATED FROM HUMAN PERIPHERAL BLOOD. THESE CELLS WERE THEN FROZEN ACCORDING TO AN AUTOMATICALLY CONTROLLED COOLING PROGRAM AND STORED IN LIQUID NITROGEN. AFTER THE FREEZING, THAWING AND WASHING, 63% OF THE CELLS PRESENT BEFORE CRYOPRESERVATION WERE RECOVERED. STORAGE AT 77K (-196 DEG C) DID NOT ALTER THE PERCENTAGE OF MONOCYTES (70-80%) IN THE SUSPENSIONS. THE CAPACITY OF MONOCYTES TO LYSE EAIGG (RH D-POSITIVE RED CELLS SENSITIZED IN VITRO WITH INCOMPLETE IGG ANTI-D ANTIBODIES) WAS NOT INFLUENCED BY CRYOPRESERVATION, IN CONTRAST WITH THEIR POTENCY TO PHAGOCYTIZE ZYMOSAN PARTICLES, WHICH WAS DECREASED. THE CHEMOTACTIC RESPONSE TOWARD CASEIN WAS ALSO DIMINISHED AFTER FREEZING. THERE WAS NO SIGNIFICANT DIFFERENCE IN REACTIVITY BETWEEN MONOCYTES FROZEN FOR A SHORT TIME (2-15 HR) AND THOSE FROZEN FOR A LONGER PERIOD (MORE THAN 3 MONTHS). ELECTRON MICROSCOPIC EXAMINATION SHOWED ALTERATIONS IN THE MITOCHONDRIAL STRUCTURE OF THE FROZEN CELLS.

Details

  • Original title: CRYOPRESERVATION OF HUMAN MONOCYTES.
  • Record ID : 1982-1051
  • Languages: English
  • Source: Cryobiology - vol. 18 - n. 4
  • Publication date: 1981/08

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