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CRYOPRESERVATION OF OIL PALM SOMATIC EMBRYOS: IMPORTANCE OF THE FREEZING PROCESS.

Author(s) : ENGELMANN F., DEREUDDRE J.

Type of article: Article

Summary

CRYOPRESERVATION OF OIL PALM SOMATIC EMBRYOS WAS CARRIED OUT USING ONE-STEP RAPID FREEZING (200 K/MIN) OR TWO-STEP FREEZING, IE PROGRAMMED COOLING FROM 293 TO 173 K (20 TO -100 DEG C) WITH COOLING RATES RANGING BETWEEN 0.5 AND 40 K/MIN FOLLOWED BY IMMERSION IN LIQUID NITROGEN. THE SURVIVAL AND RECOVERY RATES, AS WELL AS THE RECOVERY INTENSITY, WERE GREATER FOR COOLING RATES EQUAL TO OR HIGHER THAN 5 K/MIN. WITH A SLOW COOLING RATE THE RECOVERY IN ONE CLONE INCREASED WHEN THE PREFREEZING TEMPERATURE WAS DECREASED FROM 253 K (-20 DEG C) TO 173 K.

Details

  • Original title: CRYOPRESERVATION OF OIL PALM SOMATIC EMBRYOS: IMPORTANCE OF THE FREEZING PROCESS.
  • Record ID : 1989-1376
  • Languages: English
  • Source: Cryo-Letters - vol. 9 - n. 4
  • Publication date: 1988
  • Available in the IIR library

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