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Cryopreserved human haematopoietic stem cells retain engraftment potential after extended (5-14 years) cryostorage.

Author(s) : SPURR E. E., WIGGINS N. E., MARSDEN K. A., et al.

Type of article: Article

Summary

Harvesting of stem cells during the early phases of treatment with no immediate intention to perform a stem cell transplant is becoming increasingly common. Such "insurance" harvests are often stored for many years before being needed for transplant in a subsequent relapse. The effect of long-term cryostorage (5-14 years) on the viability and functional capacity of haematopoietic stem cells (HSCs) was investigated in 40 bone marrow and peripheral blood harvests using standard in vitro methods, the colony forming unit-granulocyte/macrophage (CFU-GM) assay and a single platform viable CD34+ cell absolute count by flow cytometry. It appears that HSC collections can remain adequate for safe transplantation after up to 14 years of cryostorage. However, as deterioration of HSC quality and viability may occur, some precautions may be warranted, i.e. harvesting higher than normal numbers of HSCs in collections intended for long-term storage and repeating in vitro assays after long-term storage prior to transplantation.

Details

  • Original title: Cryopreserved human haematopoietic stem cells retain engraftment potential after extended (5-14 years) cryostorage.
  • Record ID : 2003-2047
  • Languages: English
  • Source: Cryobiology - vol. 44 - n. 3
  • Publication date: 2002/06

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