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Cryopreservation of rat blastocysts: a comparative study of different cryoprotectants and freezing/thawing methods.

Author(s) : STEIN A., FISCH B., TADIR Y., OVADIA J., KRAICER P. F.

Type of article: Article

Summary

Different freezing methods for rat embryos are investigated; different temperatures (-30 or -80 deg C) and cryoprotectants are applied. The thawing methods are also described, along with the obtained survival rates. The best results were obtained with -80 deg C followed by rapid cooling. The cryoprotectants were glycerol, DMSO and propanediol/sucrose.

Details

  • Original title: Cryopreservation of rat blastocysts: a comparative study of different cryoprotectants and freezing/thawing methods.
  • Record ID : 1994-1889
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 2
  • Publication date: 1993/04

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