ShareCryopreservation of rat blastocysts: a comparative study of different cryoprotectants and freezing/thawing methods.
Author(s) : STEIN A., FISCH B., TADIR Y., OVADIA J., KRAICER P. F.
Type of article: Article
Summary
Different freezing methods for rat embryos are investigated; different temperatures (-30 or -80 deg C) and cryoprotectants are applied. The thawing methods are also described, along with the obtained survival rates. The best results were obtained with -80 deg C followed by rapid cooling. The cryoprotectants were glycerol, DMSO and propanediol/sucrose.
Details
- Original title: Cryopreservation of rat blastocysts: a comparative study of different cryoprotectants and freezing/thawing methods.
- Record ID : 1994-1889
- Languages: English
- Source: Cryobiology - vol. 30 - n. 2
- Publication date: 1993/04
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Thawing; Cryoprotectant; Rat; Survival; Embryo; Cryopreservation; Freezing
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