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Development of whole and demi-embryos of mice in culture and in vivo after supercooled storage.

Author(s) : FUKU E., FISER P. S., MARCUS G. J., SASADA H., DOWNEY B. R.

Type of article: Article

Summary

Demi-embryos and intact mouse embryos were cultured to the blastocyst stage, stored at -5 deg C for 48 hours, then cultured for 24 hours and transferred into pseudopregnant recipients. Supercooled storage did not impair the developmental potential of whole or demi-embryos in vitro, nor was there a difference between whole and demi-embryos with respect to growth in vitro. Similarly, there was no effect of supercooling on development of intact or demi-embryos after transfer into pseudopregnant recipient mice, but fewer recipients of demi-embryos remained pregnant.

Details

  • Original title: Development of whole and demi-embryos of mice in culture and in vivo after supercooled storage.
  • Record ID : 1994-2560
  • Languages: English
  • Source: Cryobiology - vol. 30 - n. 6
  • Publication date: 1993/12

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