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Effect of cooling and cryopreservation on sperm motility and morphology of several species of marsupial.

Author(s) : TAGGART D. A., LEIGH C. M., STEELE V. R., BREED W. G., TEMPLE-SMITH P. D., PHELAN J.

Type of article: Article

Summary

The cryoprotectant used was a Tris-citrate-fructose-egg yolk-glycerol diluent. Sperm motility in the fat-tailed dunnart was retained for up to six days when cooled to 4 deg C. In all species except the fat-tailed dunnart, reinitiation of motility following cryopreservation occurred across a range of glycerol concentrations (4-17%). Cryoprotectant containing 6 and/or 8% glycerol resulted in little change of motility rating or the percentage of live sperm after thawing. The unusual structural and motility characteristics of dunnart spermatozoa may account for the lack of success of sperm cryopreservation in this species.

Details

  • Original title: Effect of cooling and cryopreservation on sperm motility and morphology of several species of marsupial.
  • Record ID : 1997-2474
  • Languages: English
  • Source: Reprod. Fertil. Dev. - vol. 8 - n. 4
  • Publication date: 1996
  • Document available for consultation in the library of the IIR headquarters only.

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