Successful recovery of motility and fertility of cryopreserved Cane toad (Bufo marinus) sperm.
Author(s) : BROWNE R. K., CLULOW J., MAHONY M., CLARK A.
Type of article: Article
Summary
The recent decline and extinction of amphibian species is a worldwide phenomenon without an identified cause or solution. Assisted reproductive technologies, including sperm cryopreservation, are required to manage endangered amphibian species and preserve their genetic diversity. High rates of recovery of motility and fertilizing capacity were observed with 15% DMSO and 20% glycerol respectively. Motility and fertilization rates were similar with DMSO but diverged with glycerol as cryoprotectant. The data demonstrate the feasibility of using sperm cryopreservation with amphibian species.
Details
- Original title: Successful recovery of motility and fertility of cryopreserved Cane toad (Bufo marinus) sperm.
- Record ID : 1999-3884
- Languages: English
- Source: Cryobiology - vol. 37 - n. 4
- Publication date: 1998/12
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Thawing; Sperm; Cryoprotectant; Animal; Amphibian; Survival; Cryopreservation
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Pellet-freezing spermatozoa of two marsupials: ...
- Author(s) : MOLINIA F. C., RODGER J. C.
- Date : 1996
- Languages : English
- Source: Reprod. Fertil. Dev. - vol. 8 - n. 4
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Effect of cooling and cryopreservation on sperm...
- Author(s) : TAGGART D. A., LEIGH C. M., STEELE V. R., BREED W. G., TEMPLE-SMITH P. D., PHELAN J.
- Date : 1996
- Languages : English
- Source: Reprod. Fertil. Dev. - vol. 8 - n. 4
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RELATIONSHIP BETWEEN FINAL TEMPERATURE, THAW RA...
- Author(s) : CHANDLER J. E.
- Date : 1984/08
- Languages : English
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EFFECT OF CRYOPROTECTIVE DILUENT AND METHOD OF ...
- Author(s) : PONTBRIAND D.
- Date : 1989
- Languages : English
- Source: Cryobiology - vol. 26 - n. 4
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Cryopreservation of marmoset sperm (Callithrix ...
- Author(s) : MORRELL J. M.
- Date : 1997/01
- Languages : English
- Source: Cryo-Letters - vol. 18 - n. 1
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