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EXAMINATION OF METHODS FOR THE DEEP FREEZING OF GOAT SEMEN.

Author(s) : RITAR A. J., BALL P. D., O'MAY P. J.

Type of article: Article

Summary

THE OPTIMUM FREEZING REGIME WAS ATTAINED BY HOLDING STRAWS IN VAPOUR 40 MM ABOVE LIQUID NITROGEN FOR 30 SECONDS OR LONGER, FOLLOWED BY PLUNGING INTO LIQUID NITROGEN. STRAWS EXPOSED TO VAPOUR FOR ONLY 10 SECONDS DID NOT COOL SUFFICIENT-LY BEFORE PLUNGING, AND CELL VIABILITY WAS SERIOUSLY IMPAIRED. INITIATION OF ICE CRYSTALLIZATIONAND THE PATTERN OF COOLING OF SEMEN DEPENDED ON SIZE OF STRAWS AND THE TYPE OF RACK USED TO HOLD STRAWS IN VAPOUR DURING FREEZING. THE COOLING CURVES DIFFERED MARKEDLY FOR SEMEN FROZEN IN STRAWS IN LIQUID NITROGEN VAPOUR AND PELLETS ON DRY ICE. VIABILITY OF SPERMATOZOA WAS BETTER FOR THE PELLET-FROZEN SEMEN.

Details

  • Original title: EXAMINATION OF METHODS FOR THE DEEP FREEZING OF GOAT SEMEN.
  • Record ID : 1991-0423
  • Languages: English
  • Source: Reprod. Fertil. Dev. - vol. 2 - n. 1
  • Publication date: 1990
  • Document available for consultation in the library of the IIR headquarters only.

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