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Effect of dimethylsulfoxide (DMSO) and hydroxyethyl starch (HES) in the preservation of fractionated human marrow cells.

Author(s) : LUO K., WU G., WANG Q., SUN Y., LIU H.

Type of article: Article

Summary

A lower initial temperature rise and a delayed plateau phase in the DMSO-HES-albumin mixture in comparison with those in the DMSO/serum were observed using a programmed procedure without heat fusion compensation. Plateau phase in the mixture could also be shortened with increased liquid nitrogen to minimize the transition phase duration. However, the method of mononuclear cells immersed in a -80 deg C freezer and stored in liquid nitrogen is simple and easily standardized. Additionally, widely used lactated Ringer's solution instead of Normosol-R can be employed to prepare the mixture for the preservation of fractionated cells.

Details

  • Original title: Effect of dimethylsulfoxide (DMSO) and hydroxyethyl starch (HES) in the preservation of fractionated human marrow cells.
  • Record ID : 1995-3237
  • Languages: English
  • Source: Cryobiology - vol. 31 - n. 4
  • Publication date: 1994/08

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