ShareUNFRACTIONATED HUMAN MARROW CELL CRYOPRESERVATION USING DIMETHYLSULFOXIDE AND HYDROXYETHYL STARCH.
Author(s) : STIFF P. J.
Type of article: Article
Summary
UNFRACTIONATED BONE MARROW (BM) CELLS WERE CRYOPRESERVED IN 1-TO 2-ML ALIQUOTS USING A MIXTURE CONTAINING BOTH 5% DMSO AND 6% HYDROXYETHYL STARCH (HES) IN AN ATTEMPT TO INCREASE THE VIABLE CELL YIELD AND REDUCE THE CLUMPING AFTER THAWING, OBSERVED WHEN 10% DMSO IS USED ALONE. IT APPEARS THAT THE DMSO/HES MIXTURE ALLOWS FOR A SIMPLIFIED BM CRYOPRESERVATION TECHNIQUE THAT NOT ONLY ASSURES EXCELLENT RECOVERY OF CFU-CS AND ELIMINATES CLUMPING UPON THAWING, BUT ALSO DOES NOT REQUIRE EITHER THE USE OF A RATE-CONTROLLED FREEZER OR LIQUID NITROGEN TEMPERATURES FOR STORAGE UP TO A YEAR.
Details
- Original title: UNFRACTIONATED HUMAN MARROW CELL CRYOPRESERVATION USING DIMETHYLSULFOXIDE AND HYDROXYETHYL STARCH.
- Record ID : 1983-2011
- Languages: English
- Source: Cryobiology - vol. 20 - n. 1
- Publication date: 1983
Links
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cryoprotectant; Cell; DMSO; Survival; Bone; Marrow; Cryopreservation
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- Author(s) : LAZNICKA M., HERMANOVA E., PRUSAK J.
- Date : 1984
- Languages : English
- Source: Cryo-Letters - vol. 5 - n. 4
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- Author(s) : STIFF P. J.
- Date : 1983
- Languages : English
- Source: Ann. N. Y. Acad. Sci. - vol. 411
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- Author(s) : LIU Z.
- Date : 1989/10/11
- Languages : English
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- Author(s) : GOL'CEV A. N.
- Date : 1985
- Languages : Russian
- Source: Kriobiologija - n. 3
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- Author(s) : WANG S. Y.
- Date : 1987
- Languages : English
- Source: Cryobiology - vol. 24 - n. 3
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