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UNFRACTIONATED HUMAN MARROW CELL CRYOPRESERVATION USING DIMETHYLSULFOXIDE AND HYDROXYETHYL STARCH.

Author(s) : STIFF P. J.

Type of article: Article

Summary

UNFRACTIONATED BONE MARROW (BM) CELLS WERE CRYOPRESERVED IN 1-TO 2-ML ALIQUOTS USING A MIXTURE CONTAINING BOTH 5% DMSO AND 6% HYDROXYETHYL STARCH (HES) IN AN ATTEMPT TO INCREASE THE VIABLE CELL YIELD AND REDUCE THE CLUMPING AFTER THAWING, OBSERVED WHEN 10% DMSO IS USED ALONE. IT APPEARS THAT THE DMSO/HES MIXTURE ALLOWS FOR A SIMPLIFIED BM CRYOPRESERVATION TECHNIQUE THAT NOT ONLY ASSURES EXCELLENT RECOVERY OF CFU-CS AND ELIMINATES CLUMPING UPON THAWING, BUT ALSO DOES NOT REQUIRE EITHER THE USE OF A RATE-CONTROLLED FREEZER OR LIQUID NITROGEN TEMPERATURES FOR STORAGE UP TO A YEAR.

Details

  • Original title: UNFRACTIONATED HUMAN MARROW CELL CRYOPRESERVATION USING DIMETHYLSULFOXIDE AND HYDROXYETHYL STARCH.
  • Record ID : 1983-2011
  • Languages: English
  • Source: Cryobiology - vol. 20 - n. 1
  • Publication date: 1983

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