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Effects of hydroxyapatite nanoparticles on devitrification crystallization of different kinds of cryoprotectants.

[In Chinese. / En chinois.]

Author(s) : LU F., LIU B., LI W.

Type of article: Article

Summary

To study the effects of Hydroxyapatite (HA) nanoparticles on devitrification of different cryoprotectant solutions, the crystallization of Glycerol(60%,w/w) and PEG-600(50%,w/w) solutions with HA nanoparticles of different particle size(20nm?40nm?60nm)and concentrations(0.1%?0.5%)were observed under a cryomicroscope. Experimental results showed that the morphology of Glycerol and PEG-600 solutions is dendritic and spheric respectively, and both structures are not changed by adding nanoparticles. The ice fraction of Glycerol solution containing 0.5% HA with the particle size of 60nm is 1/7 of that of Glycerol solution without nanoparticles. The ice fraction of PEG-600 solution increased drastically between -64°C and -52°C, and the ice fraction of PEG-600 solution containing 0.5% HA with the particle size of 40nm increased by 90%. Ice growth rate has two maximum normally, however, there is only one maximum in Glycerol solution containing 0.5% HA with the particle size of 40nm, PEG-600 solution with the particle size of 60nm, and PEG-600 solution without nanoparticles.

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Pages: 18-23

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