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EFFECTS OF PRECRYOPRESERVATION CULTURE ON SURVIVAL OF RAT ISLETS TRANSPLANTED AFTER SLOW COOLING AND RAPID THAWING.

Author(s) : WARNOCK G. L., RAJOTTE R. V.

Type of article: Article

Summary

QUANTITIES OF 2,500 WISTAR-FURTH RAT ISLETS WERE ALLOCATED TO EACH OF 4 GROUPS (N = 8 EACH): GROUP 1, FRESHLY ISOLATED ; GROUP 2, 48 HR IN IN VITRO CULTURE ; GROUP 3, CRYOPRESERVATION ; GROUP 4, CRYOPRESERVATION AFTER 48 HR IN CULTURE. ISLETS WERE FROZEN SLOWLY AT 0.25 K/MIN AND THAWED RAPIDLY AT 200 K/MIN. ISLET RECOVERY WAS 97% AFTER CULTURE, 95% AFTER CRYOPRESERVATION, AND 94% AFTER CULTURE-THEN CRYOPRESERVATION. THE RESULTS SHOW THAT CRYOPRESERVATION PRESERVES FRESHLY ISOLATED OR CULTURED ISLETS THAT REVERSE THE INDICES OF DIABETES AFTER IMPLANTATION. TISSUE CULTURE NEITHER IMPROVES THE RECOVERY OF RODENT ISLETS CRYOPRESERVED WITH THESE METHODS NOR OPTIMIZES ISLET GRAFT FUNCTION.

Details

  • Original title: EFFECTS OF PRECRYOPRESERVATION CULTURE ON SURVIVAL OF RAT ISLETS TRANSPLANTED AFTER SLOW COOLING AND RAPID THAWING.
  • Record ID : 1990-0852
  • Languages: English
  • Source: Cryobiology - vol. 26 - n. 2
  • Publication date: 1989

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