ShareSUCCESSFUL LONG TERM CRYOPRESERVATION OF NEONATAL RAT ISLET TISSUE.
Author(s) : PAYNE W. D.
Type of article: Article
Summary
NEONATAL RAT PANCREATIC TISSUE WAS FROZEN TO 77 K (-196 DEG C) USING DIMETHYLSULFOXIDE AS A CRYOPROTECTANT AND A SLOW FREEZING RATE TO 203 K (-70 DEG C)FOLLOWED BY IMMERSION IN LIQUID NITROGEN. RAPID THAWING WAS USED. VIABILITY WAS DEMONSTRATED BY SUCCESSFUL TRANSPLANTATION TO STREPTOZOTOCIN-INDUCED DIABETIC RECIPIENTS. LONG-TERM PRESERVATION, UP TO 4 WEEKS, DID NOT DEMONSTRABLY AFFECT VIABILITY.
Details
- Original title: SUCCESSFUL LONG TERM CRYOPRESERVATION OF NEONATAL RAT ISLET TISSUE.
- Record ID : 1984-1241
- Languages: English
- Source: Cryobiology - vol. 20 - n. 2
- Publication date: 1983
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Cryoprotectant; Tissue; Rat; Survival; Pancreas; Cryopreservation
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- Author(s) : WARNOCK G. L., RAJOTTE R. V.
- Date : 1989
- Languages : English
- Source: Cryobiology - vol. 26 - n. 2
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Cryopreservation of rat islets of Langerhans: a...
- Author(s) : RICH S. J., SWIFT S., THIRDBOROUGH S. M., RUMFORD G., JAMES R. F. L., LONDON N. J. M.
- Date : 1993/08
- Languages : English
- Source: Cryobiology - vol. 30 - n. 4
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MORPHOLOGICAL INVESTIGATIONS INTO THE STRUCTURE...
- Author(s) : MEZZOGIORNO V., ESPOSITO V.
- Date : 1984
- Languages : English
- Source: Cryobiology - vol. 21 - n. 3
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EFFECT OF THE POLYMERIC CRYOPROTECTANT DEXTRAN ...
- Author(s) : KUIJPERS G. A.
- Date : 1983
- Languages : English
- Source: J. Microsc. - vol. 129 - n. 2
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COLD STORAGE PRESERVATION OF ISLET AND PANCREAS...
- Author(s) : SUTHERLAND D. E. R.
- Date : 1983
- Languages : English
- Source: Cryobiology - vol. 20 - n. 2
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