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Evaluation of cryopreserved cardiovascular allograft viability using real time quantitative PCR.

[In Japanese. / En japonais.]

Author(s) : NISHIZAKI K., NAGASAKA S., TANIGUCHI S.

Type of article: Article

Summary

In this study, the authors quantitatively investigated the viability of a cryopreserved allograft artery by measuring mRNA level of VEGF (vascular endothelial growth factor), HGF (hepatocyte growth factor) and b-FGF (basic fibroblast growth factor), which was associated with regeneration either with or without immunosuppression using the real time reverse transcription polymerase chain reaction (RT-PCR). The authors investigated the durability and viability of the xenograft after transplantation with morphologic analysis and the possibility of clinical use of cryopreserved xenogeneic heart valves instead of allogeneic valves. The authors can use high quality xenograft heart valves. The construction and the median smooth muscles of the aortic wall and the endothelial cells in cryopreserved xenografts were not maintained. But cryopreserved xenograft heart valves were able to maintain more fibroblasts than fresh xenogeneic heart valves at 1 month after transplantation. It was unclear whether maintenance of the fibroblasts in the cryopreserved xenograft heart valves might bring improvement of durability, especially since cryopreserved xenografts maintained tissue integrity compared with fresh xenografts.

Details

  • Original title: [In Japanese. / En japonais.]
  • Record ID : 2004-2532
  • Languages: Japanese
  • Source: Low Temperature Medicine - vol. 28 - n. 2
  • Publication date: 2002/06
  • Document available for consultation in the library of the IIR headquarters only.

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