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Functional assessment of human femoral arteries after cryopreservation.

Author(s) : RENDAL-VÁZQUEZ M. E., RODRÍGUEZ-CABARCOS M., FERNÁNDEZ-MALLO R. O., et al.

Type of article: Article

Summary

An established method for the cryopreservation of human femoral arteries for subsequent transplantation as allografts has been studied with a particular focus on preservation of smooth muscle and endothelium. Human femoral arteries (HFAs) were harvested from multi-organ donors. Two groups were established; a control group of unfrozen HFAs and a group of cryopreserved HFAs. Cryopreservation was performed using RPMI solution containing dimethyl sulfoxide and the rate of cooling was 1 °C/min to -40 °C and faster thereafter until -150 °C was reached. The contraction and relaxation responses of unfrozen and frozen/thawed arteries were assessed by measurement of the isometric force generated by the HFAs in an organ bath. After thawing the maximal contractile response to noradrenaline was 43% of the response of unfrozen HFAs. The endothelium-independent response to sodium nitroprusside was not altered, whereas the endothelium-dependent relaxation response to acetylcholine was slightly altered. The method used provided limited preservation of the contractility of human femoral arteries, and good preservation of both endothelium-independent and endothelium-dependent relaxation responses.

Details

  • Original title: Functional assessment of human femoral arteries after cryopreservation.
  • Record ID : 2005-0990
  • Languages: English
  • Source: Cryobiology - vol. 49 - n. 1
  • Publication date: 2004/08

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