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INFLUENCE OF CARBOHYDRATES, COOLING AND RAPID FREEZING ON VIABILITY OF BOVINE NON-MATURATED OOCYTES OR 1-CELL FERTILIZED EGGS.

Author(s) : HEYMAN Y.

Type of article: Article

Summary

BOVINE NON-MATURATED OOCYTES AND FERTILIZED 1-CELL EGGS WERE USED TO STUDY THE INFLUENCE OF CARBOHYDRATES, COOLING AND RAPID FREEZING ON VIABILITY. OOCYTES WERE MORE SENSITIVE TO SUCROSE TREATMENT AND SHRINKAGE THAN FERTILIZED EGGS. IN VITRO MATURATION OF OOCYTES WAS SIGNIFICANTLY REDUCED AFTER COOLING AND VERY FEW OOCYTES (6%) SURVIVED FREEZING. ON THE OTHER HAND, 1-CELL EMBRYOS WERE SUCCESSFULLY FROZEN IN LIQUID NITROGEN AND 34% CLEAVED IN VITRO AFTER THAWING.

Details

  • Original title: INFLUENCE OF CARBOHYDRATES, COOLING AND RAPID FREEZING ON VIABILITY OF BOVINE NON-MATURATED OOCYTES OR 1-CELL FERTILIZED EGGS.
  • Record ID : 1987-0380
  • Languages: English
  • Source: Cryo-Letters - vol. 7 - n. 3
  • Publication date: 1986
  • Document available for consultation in the library of the IIR headquarters only.

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