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KINETIC APPROACHES TO CRYOPRESERVATION OF RED CELLS.

Author(s) : ROWE A. W.

Summary

INTERIM PRESERVATION OF VIABLE RED CELLS AT TEMPERATUR FREEZING IS NOW POSSIBLE WITH THE USE OF SELECTED BUFFERS AND METABOLITES. PROLONGED PRESERVATION REQUIRES: (1) A CRYOPROTECTIVE ADDITIVE ; (2) CONTROLLED COOLING ; AND (3) LOW-TEMPERATURE STORAGE. SEVERAL KINETIC APPROACHES HAVE BEEN DEVELOPED THAT UTILIZE RAPID COOLING IN EITHER EXTRACELLULAR OR INTRACELLULAR ADDITIVES. HIGH RECOVERY AND SURVIVAL OF THAWED RED CELLS HAVE BEEN ACHIEVED WITH THESE BASIC APPROACHES: (1) RAPID FREEZING WITH POLYVINYL PYRROLIDONE OR HYDROXYETHYL STARCH ; (2) DROPLET FREEZING WITH SUGARS ; AND (3) RAPID COOLING WITH LOW GLYCEROL CONCENTRATION. STUDIES INDICATE CRYOPRESERVATION OF RED CELLS BY KINETIC FREEZING REQUIRES ULTRA-LOW TEMPERATURES FOR PROLONGED PRESERVATION OF VIABILITY.

Details

  • Original title: KINETIC APPROACHES TO CRYOPRESERVATION OF RED CELLS.
  • Record ID : 1990-2143
  • Languages: English
  • Publication date: 1989/07/06
  • Source: Source: Proc. IVth int. Sch. Cryobiol. Freeze-drying, Borovets
    L19-L23; 40 ref.
  • Document available for consultation in the library of the IIR headquarters only.

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