THE MECHANISM OF STRUCTURAL AND OSMOTIC ADAPTATION OF ERYTHROCYTES TO CRYOPRESERVATION.

Summary

THE AUTHORS FOUND IN THEIR EXPERIMENTS THAT STEPWISE COLD TREATMENT OF ERYTHROCYTES WITH AN IMPERMEABLE CRYOPROTECTANT POLYETHYLENE OXIDE OF MOLECULAR WEIGHT 1500 (PEO-1500) FAVOURS THE DEVELOPMENT OF INCREASED CELL RESISTANCE TO SUBSEQUENT FREEZE-THAWING. THIS CAN BE SEEN IN A SHARP DECREASE OF HEMOLYSIS AND OSMOTIC FRAGILITY OF ERYTHROCYTES AFTER TRANSFER TO MEDIA OF PHYSIOLOGICAL TONICITY, WHEN COMPARED TO CELLS TREATED AT ROOM TEMPERATURE.

Details

  • Original title: THE MECHANISM OF STRUCTURAL AND OSMOTIC ADAPTATION OF ERYTHROCYTES TO CRYOPRESERVATION.
  • Record ID : 1990-2145
  • Languages: English
  • Publication date: 1989/07/06
  • Source: Source: Proc. IVth int. Sch. Cryobiol. Freeze-drying, Borovets
    L31-L33; 4 fig.; 1 tabl.
  • Document available for consultation in the library of the IIR headquarters only.