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Quantitative method for detecting Listeria monocytogenes in meat products.

Enumeración rápida de Listeria monocytogenes en productos cárnicos mediante PCR a tiempo real.

Author(s) : RODRÍGUEZ-LÁZARO D., JOFRÉ A., AYMERICH T., et al.

Type of article: Article

Summary

The authors describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification and real time PCR. It can detect as few as 100 cfu/g and quantify as few as 1000 cfu/g, with excellent accuracy compared with the plate count method. Therefore, it is a promising alternative for the detection of Listeria monocytogenes in meat products.

Details

  • Original title: Enumeración rápida de Listeria monocytogenes en productos cárnicos mediante PCR a tiempo real.
  • Record ID : 2005-1340
  • Languages: Spanish
  • Source: Acta Alimentaria - vol. 41 - n. 357
  • Publication date: 2004/10

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