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QUICK-FREEZE FIXATION AND FREEZE-DRYING OF ISOLATED RAT PANCREATIC ISLETS: APPLICATION TO THE ULTRASTRUCTURAL LOCALIZATION OF INORGANIC PHOSPHATE IN THE PANCREATIC BETA CELL.

Author(s) : DUDEK R. W., BOYNE A. F., FREINKEL N.

Type of article: Article

Summary

A MECHANICAL QUICK-FREEZE ASSEMBLY AND ACOULTER-TERRACIO FREEZE-DRY APPARATUS WERE SUCCESSFULLY COUPLED TO OBTAIN HIGH QUALITY UNTRASTRUCTURAL PRESERVATION OF PANCREATIC BETA CELLS IN A SIMPLE AND DEPENDABLE MANNER. EXCEPT FOR OBVIOUS SHRINKAGE SPACES, MORPHOLOGICAL RELATIONSHIPS AT THE TISSUE, CELLULAR, AND SUBCELLULAR LEVELS WERE ALL INTACT. TWO CLEARLY DEFINED POPULATIONS OF MITOCHONDRIA WERE FOUND. ONE GROUP OF VERY DARK MITOCHONDRIA AND A SECOND GROUP OF MITOCHONDRIA HAD LIGHT MATRICES WITH PROMINENT CRISTAE. THE COMBINED QUICK-FREEZE FIXATION AND FREEZE-DRYING WAS APPLIED TO REEVALUATE THE ULTRASTRUCTURAL LOCALIZATION OF INORGANIC PHOSPHATE THAT HAD BEEN PRECIPITATED WITH LEAD IN THE BETA CELLS OF PANCREATIC ISLETS.

Details

  • Original title: QUICK-FREEZE FIXATION AND FREEZE-DRYING OF ISOLATED RAT PANCREATIC ISLETS: APPLICATION TO THE ULTRASTRUCTURAL LOCALIZATION OF INORGANIC PHOSPHATE IN THE PANCREATIC BETA CELL.
  • Record ID : 1982-1782
  • Languages: English
  • Source: J. Histochem. Cytochem. - vol. 29 - n. 2
  • Publication date: 1981
  • Document available for consultation in the library of the IIR headquarters only.

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