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SPERMATOZOA EVALUATION AND CRYOPRESERVATION IN PELLETS FORM OF < BAGRE >, RHAMDIA HILARII, (VALENCIENNES, 1840) SEMEN.

[In Portuguese. / En portugais.]

Author(s) : FOGLI DA SILVEIRA W., KAVAMOTO E. T., NARAHARA M. Y.

Type of article: Article

Summary

THE SPERMATIC CHARACTERISTICS AND THE CRYOPRESERVATION OF 10 AND 6 < BAGRES >, RHAMDIA HILARII SPERM RESPECTIVELY, WERE STUDIED WITH THE PURPOSE TO MAINTAIN A SEMEN FROZEN BANK FOR ARTIFICIAL FERTILIZATION OF FRESHWATER FISHES. THE SEMEN OF EACH MALE, MIXED WITH EXTENDER AT RATIO OF 1:3, WAS FROZEN IN PELLETS FORM ON DRY ICE: 194 K (-79 DEG C) AND STORED IN LIQUID NITROGEN: 77 K (-196 DEG C). SAMPLES THAWED AND EXAMINED UNDER PHASE-CONTRAST MICROSCOPY PRESENTED AN AVERAGE OF 31.94% LIVE SPERMATOZOA. SPERMATOZOA SHOWED 30% MOTILITY 36 MONTHS AFTERFREEZING.

Details

  • Original title: [In Portuguese. / En portugais.]
  • Record ID : 1988-0395
  • Languages: Portuguese
  • Source: Bol. Inst. Pesca - vol. 12 - n. 4
  • Publication date: 1985
  • Document available for consultation in the library of the IIR headquarters only.

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