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SUSCEPTIBILITY OF HUMAN T CELLS, T-CELL SUBSETS, AND B CELLS TO CRYOPRESERVATION.

Author(s) : VENKATARAMAN M., WESTERMAN M. P.

Type of article: Article

Summary

THE AUTHORS HAVE STUDIED THE NUMBER OF FUNCTIONALLY ACTIVE T AND B LYMPHOCYTES AFTER CRYOPRESERVATION. THE RESULTS WERE COMPARED TO THOSE OBTAINED FROM SIMILAR TYPES OF CELLS FRACTIONATED FROM FRESH AND FROM CRYOPRESERVED MONONUCLEAR CELLS. THE NUMBER OF VIABLE T CELLS RECOVERED AFTER CRYOPRESERVATION WAS SIGNIFICANTLY LOWER. THE T CELLS RECOVERED AFTER CRYOPRESERVATION SHOWED SIGNIFICANTLY REDUCED BLASTOGENIC ACTIVITY. THE FUNCTIONAL ABILITIES OF B CELLS WERE NOT AFFECTED REGARDLESS OF WHETHER PURIFICATION WAS DONE BEFORE OR AFTER CRYOPRESERVATION. THE DAMAGE CAN BE MINIMIZED BY REVERSING THE SEQUENCE OF T-CELL ISOLATION AND FREEZING.

Details

  • Original title: SUSCEPTIBILITY OF HUMAN T CELLS, T-CELL SUBSETS, AND B CELLS TO CRYOPRESERVATION.
  • Record ID : 1987-0763
  • Languages: English
  • Source: Cryobiology - vol. 23 - n. 3
  • Publication date: 1986

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