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Comparative studies on in vitro reactivity of fresh and cryopreserved pig lymphocytes.

Author(s) : KOCH E., LARAK M., ELLENDORFF F.

Type of article: Article

Summary

Peripheral blood mononuclear cells (PBMC) were frozen and stored in liquid nitrogen. Following thawing 74.7 more or less 2.6% of cells were recovered of which 94.5 more or less 0.9% were viable. Irrespective of storage time and cell donor, free intracellular calcium ion in frozen-thawed PBMC was significantly lower when compared to fresh cells. In addition, cryopreserved PBMC only weakly responded with an increase of free intracellular calcium ion after stimulation by various concentrations of phytohemagglutinin (PHA). Following activation by PHA for 4 days fresh lymphocytes incorporated significantly more (3H)thymidine than frozen PBMC. A similar difference in proliferation rates between fresh and frozen-thawed PBMC was observed in one-way mixed lymphocyte cultures (MLC), while the spontaneous incorporation of radiolabel was unchanged in frozen stored cells.

Details

  • Original title: Comparative studies on in vitro reactivity of fresh and cryopreserved pig lymphocytes.
  • Record ID : 1992-3064
  • Languages: English
  • Source: Cryobiology - vol. 28 - n. 5
  • Publication date: 1991/10

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