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CRYOPRESERVATION OF HUMAN LYMPHOCYTES AND STEM CELLS (CFU-C) IN LARGE UNITS FOR CANCER THERAPY. A REPORT BASED ON THE DATA OF MORE THAN 400 FROZEN UNITS.

Author(s) : SCHEIWE M. W.

Type of article: Article

Summary

A CRYOPRESERVATION PROCEDURE WAS DEVELOPED WHICH PROVED TO BE EFFECTIVE FOR LARGE VOLUMES OF LYMPHOCYTES AND STEM CELLS (CFU-C) OBTAINED EXCLUSIVELY FROM THE PERIPHERAL BLOOD OF MAN. VIABILITY CRITERIA USED FOR LYMPHOCYTES INCLUDE DETERMINATION OF THE NUMBER OF LIVING CELLS, ABSOLUTE RECOVERY AND IMMUNOLOGIC ASSAYS. A SPECIALLY DESIGNED FREEZING PROCESS AND A REVITALIZATING METHOD USING AN ATOMIZING SYSTEM WERE APPLIED TO CELL CONCENTRATES OF PATIENTS WITH DIFFERENT TYPES OF CANCER. THE RESULTS INDICATE THAT NEARLY ALL CELL FUNCTIONS OF INTEREST ARE FULLY PRESERVED.

Details

  • Original title: CRYOPRESERVATION OF HUMAN LYMPHOCYTES AND STEM CELLS (CFU-C) IN LARGE UNITS FOR CANCER THERAPY. A REPORT BASED ON THE DATA OF MORE THAN 400 FROZEN UNITS.
  • Record ID : 1982-1053
  • Languages: English
  • Source: Cryobiology - vol. 18 - n. 4
  • Publication date: 1981/08

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