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THE EFFECT OF COLD HARDENING AND COOLING RATE ON THE SURVIVAL OF APICAL MERISTEMS OF VACCINIUM SPECIES FROZEN IN LIQUID NITROGEN.

Author(s) : REED B. M.

Type of article: Article

Summary

APICAL MERISTEMS OF TISSUE CULTURED VACCINIUM SPECIES WERE TESTED FOR THEIR ABILITY TO CRYOPRESERVE FOLLOWING COLD HARDENING. IN VITRO-GROWN PLANTS WERE COLD HARDENED/CHILLED AT ALTERNATING TEMPERATURES: 22 DEG C DAY, -1 DEG C NIGHT, FOR 0, 1, 3, 5 OR 7 WEEKS BEFORE THE MERISTEMS WEREEXCISED AND COOLED TO -35 DEG C AT 4 DIFFERENT RATES. SURVIVAL RATE FOLLOWING IMMERSION IN LIQUID NITROGEN AND RAPID THAWING VARIED GREATLY AMONG SPECIES.

Details

  • Original title: THE EFFECT OF COLD HARDENING AND COOLING RATE ON THE SURVIVAL OF APICAL MERISTEMS OF VACCINIUM SPECIES FROZEN IN LIQUID NITROGEN.
  • Record ID : 1990-1906
  • Languages: English
  • Source: Cryo-Letters - vol. 10 - n. 5
  • Publication date: 1989/09
  • Document available for consultation in the library of the IIR headquarters only.

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