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COLD ACCLIMATION AS A METHOD TO IMPROVE SURVIVAL OF CRYOPRESERVED RUBUS MERISTEMS.

Author(s) : REED B. M.

Type of article: Article

Summary

TISSUE CULTURE PLANTLETS OF RUBUS SPECTABILIS PURSH, R. IDAEUS L < HERITAGE > AND RUBUS SPP < LOGAN THORNLESS > AND < MERTON THORNLESS > WERE COLD ACCLIMATED: 25/-1 DEG C, DAY/NIGHT TEMPERATURES, FOR 1 WEEK PRECEDING EXCISION FOR CRYOPRESERVATION. MERISTEMS WERE SLOWLY FROZEN TO -40 DEG C FOLLOWED BY IMMERSION IN LIQUID NITROGEN FOR 1 HOUR. SURVIVAL IN NON-ACCLIMATED PLANTS RANGED FROM 18-41% WITH 51-67% SURVIVAL IN ACCLIMATED MERISTEMS. ACCLIMATED R. SPECTABILIS AND < MERTON THORNLESS > RETAINED THEIR HIGH SURVIVAL RATES FOLLOWING 30, 60 AND 180 DAYS OF STORAGE IN LIQUID NITROGEN.

Details

  • Original title: COLD ACCLIMATION AS A METHOD TO IMPROVE SURVIVAL OF CRYOPRESERVED RUBUS MERISTEMS.
  • Record ID : 1989-0124
  • Languages: English
  • Source: Cryo-Letters - vol. 9 - n. 3
  • Publication date: 1988/05
  • Document available for consultation in the library of the IIR headquarters only.

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