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Cryoconservation du plasma germinal de pomme sensible au froid.

Cryopreservation of cold-tender apple germplasm.

Auteurs : SEUFFERHELD M. J., STUSHNOFF C., FORSLINE P. L., et al.

Type d'article : Article

Résumé

Unlike cold-hardy apple germplasm, dormant vegetative buds from cold-tender accessions require stabilization of meristematic tissue to protect against injury during desiccation and cryopreservation. Dormant buds of six apple cultivars collected at specific intervals in 1993, 1994, and 1995 at Geneva, USA, were stabilized by encapsulation in 5% alginate, treated with step-wise inhibition of 0.5 to 1.0 M sucrose and 0.2 M raffinose solution, and desiccated with forced air at 0 °C. Sugar-alginate stabilization reduced injury during desiccation, increased cold-hardiness of the six cold-tender cultivars frozen to -30 °C, and improved recovery following cryopreservation of buds collected before optimal cold acclimation was attained. Sucrose tissue levels did not increase following stabilization treatment, but levels of glucose and fructose, and of an unknown disaccharide increased. This procedure used nontoxic cryoprotectants, and has potential to expand the scope of dormant bud cryopreservation to include cold-tender apple germplasm.

Détails

  • Titre original : Cryopreservation of cold-tender apple germplasm.
  • Identifiant de la fiche : 2001-0323
  • Langues : Anglais
  • Source : J. am. Soc. hortic. Sci. - vol. 124 - n. 6
  • Date d'édition : 11/1999
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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