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Détection des pathogènes dans des viandes et produits carnés : développement d'une technique immunofluorescente fondée sur l'adhésion à la surface.

The development of a surface adhesion immunofluorescent technique for the detection of pathogens from meat and meat products.

Auteurs : DUFFY G., LOGUE C. M., CLOAK O. M., et al.

Résumé

A novel surface adhesion immunofluorescent technique to isolate pathogens (Listeria monocytogenes, Yersinia enterocolitica, Salmonella typhimurium) from meat was developed. The method for detection of L. monocytogenes involved inoculation of minced beef samples with the pathogen. The meat sample was incubated at 30 °C for 14-18 h in a suitable enrichment broth. L. monocytogenes cells were isolated from the enriched meat sample by surface adhesion onto a polycarbonate membrane which was attached to a glass microscope slide. The Listeria cells on the membrane were subsequently visualized using immunofluorescent microscopy. The technique was demonstrated to have a detection level of log(10) 3.11 cfu/ml. The use of this rapid technique for the detection of other pathogens from meat including Y. enterocolitica and S. typhimurium is also discussed.

Détails

  • Titre original : The development of a surface adhesion immunofluorescent technique for the detection of pathogens from meat and meat products.
  • Identifiant de la fiche : 2000-3081
  • Langues : Anglais
  • Source : Factors affecting the microbial quality of meat. 4. Microbial methods for the meat industry. Concerted Action CT94-1456.
  • Date d'édition : 1996
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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