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A 31P NMR investigation of the energy charge of the housefly Musca domestica (Diptera: muscidae) during rapid cold hardening and cold shock.

Author(s) : COULSON S. J., FISHER J., BALE J. S.

Type of article: Article

Summary

Analysis of the 31P content of housefly pupae showed six phosphate signals comprising ATP, ADP, inorganic phosphate, sugar phosphate and arginine phosphate. After exposure to a lethal cold shock (direct transfer from the culture temperature of 27 deg C to -7 deg C for 2h), the cellular content of ATP and arginine phosphate increased. However, when pupae were acclimated at 0 deg C for 3h immediately prior to exposure to -7 deg C for 2h, adult emergence on return to 27 deg C was 84% (93% in the uncooled controls) and the ATP/ADP ratios remained constant. The high ATP content of both acclimated and cold shocked pupae suggests that mortality following a cold shock exposure in M. domestica cannot be attributed to a critical reduction in cellular ATP following the low temperature exposure.

Details

  • Original title: A 31P NMR investigation of the energy charge of the housefly Musca domestica (Diptera: muscidae) during rapid cold hardening and cold shock.
  • Record ID : 1993-0511
  • Languages: English
  • Source: Cryo-Letters - vol. 13 - n. 3
  • Publication date: 1992/05
  • Document available for consultation in the library of the IIR headquarters only.

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