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A NOTE ON OPTIMAL TECHNIQUES OF RAPID COOLING FOR LOW TEMPERATURE PREPARATION OF ELECTRON MICROSCOPY SPECIMENS.

Author(s) : DILLER K. R.

Type of article: Article

Summary

ANALYTICAL PREDICTIONS WERE MADE OF THE DISTRIBUTION OF TEMPERATURE AND COOLING RATE DURING RAPID CHILLING OF BIOLOGICAL SPECIMENS IN PREPARATION FOR ELECTRON MICROSCOPY. THE TEMPERATURE WAS DETERMINED IN A SEMI-INFINITE MEDIUM AS A FUNCTION OF POSITION AND TIME FOR BOTH FREEZING AND VITRIFICATION PROTOCOLS IN RESPONSE TO A STEP-WISE DECREASE IN THE SURFACE TEMPERATURE. THE ANALYSIS SIMULATES THE LIMITING CASE OF RAPID COOLING THAT COULD BE ACHIEVED BY MANIPULATION OF THE SPECIMEN BOUNDARY CONDITIONS.

Details

  • Original title: A NOTE ON OPTIMAL TECHNIQUES OF RAPID COOLING FOR LOW TEMPERATURE PREPARATION OF ELECTRON MICROSCOPY SPECIMENS.
  • Record ID : 1990-2596
  • Languages: English
  • Source: Cryo-Letters - vol. 11 - n. 1
  • Publication date: 1990/01
  • Document available for consultation in the library of the IIR headquarters only.

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