A ONE-STEP IN SITU DILUTION METHOD FOR FROZEN-THAWED BOVINE EMBRYOS.
Author(s) : LEIBO S. P.
Type of article: Article
Summary
BOVINE EMBRYOS FROZEN AND THAWED WITHIN PLASTIC ARTIFICIAL INSEMINATION STRAWS CAN BE IMMEDIATELY DILUTED IN ONE-STEP OUT OF THE PROTECTIVE COMPOUND IN SITU WITHIN THE CONTAINER IN WHICH THEY WERE FROZEN. THIS DILUTION METHOD IS SIMPLE AND YIELDS AS HIGH SURVIVAL AS MORE COMMONLY USED MULTI-STEP DILUTIONS. BECAUSE THE EMBRYO IS SEALED IN THE STRAW, THE ENTIRE DILUTION AND RECOVERY PROCESS OF FROZEN EMBRYOS CAN BE PERFORMED WITHOUT A MICROSCOPE OR OTHER LABORATORY APPARATUS.
Details
- Original title: A ONE-STEP IN SITU DILUTION METHOD FOR FROZEN-THAWED BOVINE EMBRYOS.
- Record ID : 1984-2493
- Languages: English
- Source: Cryo-Letters - vol. 4 - n. 6
- Publication date: 1983
- Document available for consultation in the library of the IIR headquarters only.
Links
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Indexing
- Themes: The influence of refrigeration on cells, tissues and organs
- Keywords: Bovine; Survival; Embryo; Cryopreservation
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