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Acid-base buffering in organ preservation solutions as a function of temperature: new parameters for comparing buffer capacity and efficiency.

Author(s) : BAICU S. C., TAYLOR M. J.

Type of article: Article

Summary

Control of acidity and preventing intracellular acidosis are recognized as critical properties of an effective organ preservation solution. Buffer capacity and efficiency are therefore important for comparing the merits of preservation fluids for optimum hypothermic storage, but these parameters are not available for the variety of organ preservation solutions of interest in transplantation today. Buffer capacity is dependent upon concentration and pH; the buffer capacity is not easily predicted for a solution containing multiple buffer species. Using standard electrometric methods to measure acid dissociation constants, this study determined the maximum and relative buffer capacities of a variety of new and commonly used hypothermic preservation solutions. The reference data obtained show that comparative buffer capacity and efficiency vary widely between the commonly used solutions. Moreover, the fluids containing zwitterionic sulfonic acid buffers such as Hepes possess superior buffering for alpha-stat pH regulation in the region of physiological importance.

Details

  • Original title: Acid-base buffering in organ preservation solutions as a function of temperature: new parameters for comparing buffer capacity and efficiency.
  • Record ID : 2003-3045
  • Languages: English
  • Source: Cryobiology - vol. 45 - n. 1
  • Publication date: 2002/08

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