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Newly developed encapsulation-dehydration protocol for plant cryopreservation.

Author(s) : SAKAI A., MATSUMOTO T., HIRAI D., et al.

Type of article: Article

Summary

A simplified and efficient encapsulation-dehydration protocol (a compromise between vitrification and encapsulation-dehydration) was presented for plant cryopreservation. During the encapsulation process, the apices precultured with 0.3 M sucrose for 16 h were osmoprotected with a mixture of 2 M glycerol plus 0.4 M sucrose for 1 h. These encapsulated apices were directly dehydrated with dry silica gel prior to a plunge into liquid nitrogen without pretreatment using 0.8 M sucrose for 16 h. This protocol produced much higher rates of recovery growth in the three plant species tested (wasabi, chrysanthemum, and mint) than those cryopreserved by the conventional encapsulation-dehydration and considerably reduced the time needed for the cryogenic procedure. This new protocol appears promising for cryopreservation of shoot apices and other explants.

Details

  • Original title: Newly developed encapsulation-dehydration protocol for plant cryopreservation.
  • Record ID : 2001-1098
  • Languages: English
  • Source: CryoLetters - vol. 21 - n. 1
  • Publication date: 2000/01
  • Document available for consultation in the library of the IIR headquarters only.

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